Journal:
Article Title: Development and Evaluation of a PCR-Based Assay for Detection of Haemobartonella felis in Cats and Differentiation of H. felis from Related Bacteria by Restriction Fragment Length Polymorphism Analysis
doi:
Figure Lengend Snippet: Amplification of bacterial 16S rRNA with a conserved primer set, fHf1 and rHf2. Lanes: 1, 2, and 3, amplified DNA products extracted from cat blood before experimental infection with H. felis, during a heavily parasitemic episode, and following treatment, respectively; 4, water-DNA preparation; 5, B. bacilliformis; 6, M. genitalium; 7, E. suis; M, 1-kb DNA size markers cut with EcoRI and HindIII.
Article Snippet: A 1-kb DNA ladder (Lambda DNA/ Eco RI and Hin dIII Markers; Promega Corp., Madison, Wis.; http://www.promega.com ) was included as a DNA size standard.
Techniques: Amplification, Infection